ycsG [2019-06-21 09:11:54]

similar to branched chain amino acids transporter

Locus: BSU04060

Isoelectric point: 10.15

Molecular weight: 41.93 kDa

Protein length: 404 aa

Gene length: 1215 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ycsH

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Other transporters
• Groups of genes → Membrane proteins

Gene

Coordinates: 457,811 459,025

Phenotypes of a mutant

• no growth with 5-oxoproline as single source of nitrogen PubMed

The protein

Protein family

• NRAMP family (according to Swiss-Prot)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: pxpA-ycsG-ycsI-pxpB-pxpC-kipR-lipC
  Description: PubMed

  Regulatory mechanism

  • KipR: repression, PubMed, in KipR regulon
  • TnrA: activation, PubMed, in TnrA regulon

  Regulation

  • induced in the presence of 5-oxoproline PubMed

Biological materials

Mutant

• MGNA-C022 (ycsG::erm), available at the NBRP B. subtilis, Japan
• BKE04060 (ycsG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGCTCCATCTATTGTTCCT, downstream forward: _UP4_TGATATAAGTGGAGCAATTA
• BKK04060 (ycsG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGCTCCATCTATTGTTCCT, downstream forward: _UP4_TGATATAAGTGGAGCAATTA

References

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